General Cell Based Assays
Eurofins Discoverx PathHunter eXpress EpoR/EpoR Dimerization Assay, 2-Plate Kit. (93-0965E3CP0M)
The PathHunter EpoR/EpoR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. erythropoietin) induced dimerization of EpoR/EpoR receptors. This product uses EFC technology. The cell line included overexpresses EpoR target protein fused to PK alongside EpoR target protein fused to EA. Upon ligand-induced dimerization of EpoR/EpoR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GLP2R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0572E2CP2M)
The PathHunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter BDKRB2 Bioassay Kit, 10-Plate. (93-0189Y2-00114)
The PathHunter BDKRB2 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. bradykinin) based activation of BDKRB2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged BDKRB2 and EA-tagged β-Arrestin-2. Activation of BDKRB2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Innovative Research Inc Rat Ttl tPA Kit
Rat Total tPA ELISA Kit - This rat tissue-type plasminogen activator (tPA) total antigen assay is intended for the quantitative determination of total tPA in rat plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. Rat tPA will bind to the affinity purified capture antibody coated on the microtiter plate. Free and complexed tPA will bind to the plate. After appropriate washing steps, monoclonal anti-rat tPA primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with the peroxidase conjugated secondary antibody. Following an additional washing step, TMB is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of rat tPA. Color development is proportional to the concentration of tPA in the samples.
MilliporeSigma BIOLOGY CHEMISCREEN153 CX3C
Life Sciences,Apoptosis & Cell Biology,ChemiSCREEN CX3CR1 Membrane Preparation
Revvity Health Sciences Inc HTRF mAb Anti-FLAG XL665-Conjugate, 5,000 Assay Points
HTRF mAb Anti-FLAG XL665-Conjugate, 5,000 Assay Points
Enzo Life Sciences CHEMILUM DE LYS HDAC/SIRT Chemiluminescent drug discovery kit (1x96wells)
The HDAC/SIRT Chemiluminescent Drug Discovery Kit is a complete assay system designed to measure histone deacetylase and sirtuin activity in cell or nuclear extracts, immunoprecipitates or purified enzymes. The HDAC Chemiluminescent Activity Assay is based on the unique CHEMILUM DE LYS Substrate and Developer combination. The CHEMILUM DE LYS system is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has three steps. First, the CHEMILUM DE LYS Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity . Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the CHEMILUM DE LYS Developer followed by Enhancer produces light. The reaction is luciferase-free Alternative name: Histone deacetylase fluorescent assay kit. Applications: Chemiluminescence, HTS Activity assay.
Eurofins Discoverx PathHunter eXpress CCR9 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0296E2CP0M)
The PathHunter CCR9 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL25) based activation of CCR9 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR9 and EA-tagged β-Arrestin-2. Activation of CCR9 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human BMP-6 ASY
The R-PLEX Human BMP-6 Assay Kit from Meso Scale Discovery is an electrochemiluminescent immunoassay for the quantitative measurement of human Bone Morphogenetic Protein 6 (BMP-6) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SECTOR Imager 6000 for high sensitivity detection The kit includes a 96-well plate coated with capture antibody detection antibody calibrator and sample diluent The assay has a range from 1 5 pg/mL to 1000 pg/mL and takes approximately 3 5 hours to complete This product can be used for biomarker research related to bone formation fracture healing and developmental disorders
Meso Scale Discovery R-PLEX Human E-Cadherin ASY
The R-Plex Human E-Cadherin Assay from Meso Scale Diagnostics is a multiplex immunoassay that measures E-Cadherin levels in human serum plasma and cell culture supernatants It utilizes electrochemiluminescence detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay allows for the quantitative measurement of E-Cadherin an important biomarker that plays a role in cell adhesion and is often monitored in cancer research With minimal hands-on time this robust and reproducible assay generates rapid results using automated instrumentation
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 10-Plate Kit. (93-0271E2CP2L)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Biochain Institute Inc Hemoglobin Assay Kit, 250 Assays/PK
BioChain's hemoglobin concentration assay kit is a simple, direct and automation-ready assay for measuring hemoglobin. The improved cyanohemiglobin method, in which the hemoglobin is converted into a uniform colored end product. The intensity of the color is directly proportional to the hemoglobin concentration in the sample. The optimized formulation exhibits high sensitivity and substantially reduces interference by substances in the raw samples.