General Cell Based Assays
Eurofins Discoverx PathHunter eXpress MRGPRE CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0438E2ACP1M)
The PathHunter MRGPRE eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of MRGPRE GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MRGPRE and EA-tagged β-Arrestin-2. Activation of MRGPRE stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 10 Reagent, 100. (93-0563R10A)
AssayComplete Cell Plating 10 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Abcam RG 108, non-nucleoside DNA methyltransferase inhibitor (DNMT), 5MG
MW 334.3 Da, Purity >99%. Novel, non-nucleoside DNA methyltransferase (DNMT) inhibitor, capable of interacting with the DNMT1 active site (IC₅₀ = 115 nM). Inhibits DNA methylation in human cancer cell lines. Reactivates several epigenetically silenced tumour suppressor genes in vitro. Capable of enhancing the efficiency of induced pluripotent stem cell (iPS) generation.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter AGTRL1 Bioassay Kit, 10-Plate. (93-0250Y2-00122)
The PathHunter AGTRL1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-2. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress MC4R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0211E3CP0L)
The PathHunter MC4R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-MSH) based activation of MC4R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MC4R and EA-tagged β-Arrestin-2. Activation of MC4R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NPY1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0397E2CP0L)
The PathHunter NPY1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. peptide YY) based activation of NPY1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NPY1R and EA-tagged β-Arrestin-2. Activation of NPY1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Echelon Biosciences Research Labs PI(4,5)P2 DiC16 1 mg
Phosphatidylinositol 4,5-bisphosphate diC16 (PI(4,5)P2 diC16) is a synthetic, purified dipalmitoyl PI(4,5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions, PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues and is also a substrate for class I phosphoinositide 3-kinase (PI3-K). This item is non returnable
Eurofins Discoverx PathHunter eXpress CCR5 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0224E2CP0L)
The PathHunter CCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL3/MIP-1α) based activation of CCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR5 and EA-tagged β-Arrestin-2. Activation of CCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-2 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-2 Assay Kit is a sensitive immunoassay for detecting and quantifying mouse interleukin 2 (IL-2) in various biological fluids It uses MSD s electrochemiluminescent detection technology and includes plates with capture antibodies detection antibodies and calibrators The assay has a sensitivity of 0 06 pg/mL a dynamic range over 3 logs requires 50 L sample volume and is compatible with MSD platforms The kit contains all necessary reagents for measuring mouse IL-2
Eurofins Discoverx PathHunter eXpress rCRTH2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0580E2RCP2L)
The PathHunter rCRTH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin D2) based activation of rCRTH2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCRTH2 and EA-tagged β-Arrestin-2. Activation of rCRTH2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR27 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0334E2ACP1M)
The PathHunter GPR27 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR27 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR27 and EA-tagged β-Arrestin-2. Activation of GPR27 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Rat IL-6 ASY
The Meso Scale Discovery R-PLEX Rat IL-6 Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of rat interleukin 6 (IL-6) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with low sample volume requirements The assay requires 50 L or less of sample and has a testing time of 3 5 hours The lower limit of detection for rat IL-6 is 1 3 pg/mL This assay is for research use only and is not intended for diagnostic procedures It is available as a singleplex assay or as part of a multiplex panel
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 2-Plate Kit. (93-0271E2CP2M)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human ADRA2C Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human ADRA2C Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure ADRA2C (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human MIP-1 alpha (CCR1) Activated GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human MIP-1 alpha (CCR1) Stable Cell Line Activated GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure CCR1 (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.